Disposable amperometric biosensor based on nanostructured bacteriophages for glucose detection
- Authors
- Kang, Yu Ri; Hwang, Kyung Hoon; Kim, Ju Hwan; Nam, Chang Hoon; Kim, Soo Won
- Issue Date
- Oct-2010
- Publisher
- IOP PUBLISHING LTD
- Keywords
- disposable analytical system; biosensor application; bacteriophage; label-free detection
- Citation
- MEASUREMENT SCIENCE AND TECHNOLOGY, v.21, no.10
- Indexed
- SCIE
SCOPUS
- Journal Title
- MEASUREMENT SCIENCE AND TECHNOLOGY
- Volume
- 21
- Number
- 10
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/115572
- DOI
- 10.1088/0957-0233/21/10/105804
- ISSN
- 0957-0233
- Abstract
- The selection of electrode material profoundly influences biosensor science and engineering, as it heavily influences biosensor sensitivity. Here we propose a novel electrochemical detection method using a working electrode consisting of bio-nanowires from genetically modified filamentous phages and nanoparticles. fd-tet p8MMM filamentous phages displaying a three-methionine (MMM) peptide on the major coat protein pVIII (designated p8MMM phages) were immobilized on the active area of an electrochemical sensor through physical adsorption and chemical bonding. Bio-nanowires composed of p8MMM phages and silver nanoparticles facilitated sensitive, rapid and selective detection of particular molecules. We explored whether the composite electrode with bio-nanowires was an effective platform to detect the glucose oxidase. The current response of the bio-nanowire sensor was high at various glucose concentrations (0.1 mu M-0.1 mM). This method provides a considerable advantage to demonstrate analyte detection over low concentration ranges. Especially, phage-enabled bio-nanowires can serve as receptors with high affinity and specificity for the detection of particular biomolecules and provide a convenient platform for designing site-directed multifunctional scaffolds based on bacteriophages and may serve as a simple method for label-free detection.
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