Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model

Authors
Yamamoto, SakuraWada, JunKatayama, TakaneJikimoto, TakumiNakamura, MasakuniKinoshita, ShohiroLee, Kyung-MiKawabata, MasatoShirakawa, Toshiro
Issue Date
24-Sep-2010
Publisher
ELSEVIER SCI LTD
Keywords
Bifidobacterium; Mucosal vaccine; Salmonella
Citation
VACCINE, v.28, no.41, pp.6684 - 6691
Indexed
SCIE
SCOPUS
Journal Title
VACCINE
Volume
28
Number
41
Start Page
6684
End Page
6691
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/115661
DOI
10.1016/j.vaccine.2010.08.007
ISSN
0264-410X
Abstract
We developed a novel vaccine platform utilizing Bifidobacterium as an antigen delivery vehicle for mucosal immunization. Genetically modified Bifidobacterium longum displaying Salmonella-flagellin on the cell surface was constructed for the oral typhoid vaccine. The efficiency of this vaccine was evaluated in a murine model of typhoid fever. We then orally administered 2.5 x 10(7) CFU of the recombinant Bifidobacterium longum (vaccine) or parental Bifidobacterium longum, or PBS to BALB/C mice every other day for 2 weeks. After the administration, a total of 42 mice (14 mice in each group) were challenged with Salmonella Typhimurium (1.0 x 10(7) CFU/mouse). While 12 mice in the PBS group, and 9 in the parental Bifidobacterium longum group died (median survival: 14 and 25 days), only two in the vaccine group died. These data support that our genetically modified Bifidobacterium antigen delivery system offers a promising vaccine platform for inducing efficient mucosal immunity. (C) 2010 Elsevier Ltd. All rights reserved.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Lee, Kyung Mi photo

Lee, Kyung Mi
Department of Biomedical Sciences
Read more

Altmetrics

Total Views & Downloads

BROWSE