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Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model

Authors
Yamamoto, SakuraWada, JunKatayama, TakaneJikimoto, TakumiNakamura, MasakuniKinoshita, ShohiroLee, Kyung-MiKawabata, MasatoShirakawa, Toshiro
Issue Date
24-9월-2010
Publisher
ELSEVIER SCI LTD
Keywords
Bifidobacterium; Mucosal vaccine; Salmonella
Citation
VACCINE, v.28, no.41, pp.6684 - 6691
Indexed
SCIE
SCOPUS
Journal Title
VACCINE
Volume
28
Number
41
Start Page
6684
End Page
6691
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/115661
DOI
10.1016/j.vaccine.2010.08.007
ISSN
0264-410X
Abstract
We developed a novel vaccine platform utilizing Bifidobacterium as an antigen delivery vehicle for mucosal immunization. Genetically modified Bifidobacterium longum displaying Salmonella-flagellin on the cell surface was constructed for the oral typhoid vaccine. The efficiency of this vaccine was evaluated in a murine model of typhoid fever. We then orally administered 2.5 x 10(7) CFU of the recombinant Bifidobacterium longum (vaccine) or parental Bifidobacterium longum, or PBS to BALB/C mice every other day for 2 weeks. After the administration, a total of 42 mice (14 mice in each group) were challenged with Salmonella Typhimurium (1.0 x 10(7) CFU/mouse). While 12 mice in the PBS group, and 9 in the parental Bifidobacterium longum group died (median survival: 14 and 25 days), only two in the vaccine group died. These data support that our genetically modified Bifidobacterium antigen delivery system offers a promising vaccine platform for inducing efficient mucosal immunity. (C) 2010 Elsevier Ltd. All rights reserved.
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