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O-2 plasma patterning of p-type MWCNT and its application to immunosensor
- Authors
- Lee, Jun-Yong; Park, Eun Jin; Kim, Joon Hyub; Kim, Sun Guk; Lee, Cheol Jin; Kim, Min Ja; Min, Nam Ki
- Issue Date
- 1-Sep-2010
- Publisher
- ELSEVIER SCIENCE SA
- Keywords
- MWCNTs; Plasma-pattern; Immunosensors; Legionella pneumophila; ELISA
- Citation
- THIN SOLID FILMS, v.518, no.22, pp.6646 - 6649
- Indexed
- SCIE
SCOPUS
- Journal Title
- THIN SOLID FILMS
- Volume
- 518
- Number
- 22
- Start Page
- 6646
- End Page
- 6649
- ISSN
- 0040-6090
- Abstract
- An electrochemical immunosensor based on patterned plasma-functionalized multiwalled carbon nanotubes (pf-MWCNTs) working electrode for Legionella pneumophila detection is described in this paper. Polyclonal antibody was immobilized using passive adsorption and covalent immobilization on the surface of the pf-MWCNTs. Various dilutions of Legionella 19-kDa peptidoglycan-associated lipoprotein (PAL) were added onto the electrode surface and incubated. The assay was then completed using polyclonal antibody conjugated to horseradish peroxidase (HRP) as the enzyme label. 3,3',5,5'-Tetramethylbenzidine dihydrochloride (TMB)/substrate solution (H2O2) was used as the mediator/substrate system. Electrochemical detection was conducted using cyclic voltammetric at 500 mV versus silver/silver chloride (Ag/AgCl) reference electrode. L pneumophila detection of 0.16 ng ml(-1) and similar to 0.0025 ng ml(-1) was achieved respectively for passive adsorption and covalent immobilization. (C) 2010 Elsevier B.V. All rights reserved.
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Related Researcher
- Lee, Cheol Jin
- College of Engineering (School of Electrical Engineering)