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O-2 plasma patterning of p-type MWCNT and its application to immunosensor

Authors
Lee, Jun-YongPark, Eun JinKim, Joon HyubKim, Sun GukLee, Cheol JinKim, Min JaMin, Nam Ki
Issue Date
1-9월-2010
Publisher
ELSEVIER SCIENCE SA
Keywords
MWCNTs; Plasma-pattern; Immunosensors; Legionella pneumophila; ELISA
Citation
THIN SOLID FILMS, v.518, no.22, pp.6646 - 6649
Indexed
SCIE
SCOPUS
Journal Title
THIN SOLID FILMS
Volume
518
Number
22
Start Page
6646
End Page
6649
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/115705
DOI
10.1016/j.tsf.2009.12.107
ISSN
0040-6090
Abstract
An electrochemical immunosensor based on patterned plasma-functionalized multiwalled carbon nanotubes (pf-MWCNTs) working electrode for Legionella pneumophila detection is described in this paper. Polyclonal antibody was immobilized using passive adsorption and covalent immobilization on the surface of the pf-MWCNTs. Various dilutions of Legionella 19-kDa peptidoglycan-associated lipoprotein (PAL) were added onto the electrode surface and incubated. The assay was then completed using polyclonal antibody conjugated to horseradish peroxidase (HRP) as the enzyme label. 3,3',5,5'-Tetramethylbenzidine dihydrochloride (TMB)/substrate solution (H2O2) was used as the mediator/substrate system. Electrochemical detection was conducted using cyclic voltammetric at 500 mV versus silver/silver chloride (Ag/AgCl) reference electrode. L pneumophila detection of 0.16 ng ml(-1) and similar to 0.0025 ng ml(-1) was achieved respectively for passive adsorption and covalent immobilization. (C) 2010 Elsevier B.V. All rights reserved.
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공과대학 (전기전자공학부)
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