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Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins
- Authors
- Yoon, Jae-Wook; Jung, Jae-Yeon; Chung, Hyun-Jung; Kim, Mi-Ryung; Kim, Chan-Wha; Lim, Seung-Taik
- Issue Date
- Sep-2010
- Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- Keywords
- Starch granule-associated protein (SGAP); SDS-PAGE; Starch noodle; Botanical origin
- Citation
- JOURNAL OF CEREAL SCIENCE, v.52, no.2, pp.321 - 326
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CEREAL SCIENCE
- Volume
- 52
- Number
- 2
- Start Page
- 321
- End Page
- 326
- ISSN
- 0733-5210
- Abstract
- Starch granule-associated proteins (SGAPs) were extracted from various starches and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. The SDS-PAGE results showed that granule-bound starch synthase (GBSS) of approximately 60 kDa was found in most starches, except waxy-type starches. Starches exhibited the presence of proteins specific to the botanical origin, including those of 22 kDa for maize; 160 and 98 kDa for potato; 140, 115, 90, and 80 kDa for wheat. These proteins could be detected from noodles prepared with the corresponding starches. The detection of the specific proteins by SDS-PAGE may be used to identify the origin of starch incorporated in various foods and industrial products. (C) 2010 Elsevier Ltd. All rights reserved.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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