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Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles
- Authors
- Lee, Yeon-Ji; Park, Jong-Moon; Huh, Jiyoung; Kim, Minsik; Lee, Je-Sun; Palani, Arudra; Lee, Kwangyeol; Lee, Sang-Won
- Issue Date
- 20-6월-2010
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- Proteomics; Glutathione; Superparamagnetic; Nanoparticle; Separation
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.31, no.6, pp.1568 - 1572
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 31
- Number
- 6
- Start Page
- 1568
- End Page
- 1572
- ISSN
- 0253-2964
- Abstract
- The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathione-modified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/MS/MS experiments.
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