Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression
- Authors
- Yoo, Jung-Yoon; Choi, Kyung-Chul; Kang, HeeBum; Kim, Young Jun; Lee, Jeongmin; Jun, Woo Jin; Kim, Mi-Jeong; Lee, Yoo-Hyun; Lee, Ok-Hee; Yoon, Ho-Geun
- Issue Date
- 3-6월-2010
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- L(3)mbt-like 2; Histone deacetylase 3; MBT domain; Transcription
- Citation
- FEBS LETTERS, v.584, no.11, pp.2225 - 2230
- Indexed
- SCIE
SCOPUS
- Journal Title
- FEBS LETTERS
- Volume
- 584
- Number
- 11
- Start Page
- 2225
- End Page
- 2230
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/116260
- DOI
- 10.1016/j.febslet.2010.03.048
- ISSN
- 0014-5793
- Abstract
- This is the first report that L(3) mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression. Structured summary: MINT-7719975: L3MBTL2 (uniprotkb:Q969R5) and HDAC3 (uniprotkb:O15379) colocalize (MI:0403) by fluorescence microscopy (MI: 0416) MINT-7719941, MINT-7719921: L3MBTL2 (uniprotkb: Q969R5) binds (MI: 0407) to HDAC3 (uniprotkb: O15379) by pull down (MI: 0096) MINT-7719991: HDAC3 (uniprotkb: O15379) physically interacts (MI: 0915) with L3MBTL2 (uniprotkb: Q969R5) by anti bait coimmunoprecipitation (MI: 0006) MINT-7719958: L3MBTL2 (uniprotkb: Q969R5) physically interacts (MI: 0915) with HDAC3 (uniprotkb: O15379) by anti tag coimmunoprecipitation (MI: 0007) MINT-7719897: HDAC3 (uniprotkb: O15379) physically interacts (MI: 0915) with L3MBTL2 (uniprotkb: Q969R5) by two hybrid (MI: 0018) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Food and Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.