Gene cloning and characterization of a trehalose synthase from Corynebacterium glutamicum ATCC13032
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae-Kyun | - |
dc.contributor.author | Jang, Jun-Hyuck | - |
dc.contributor.author | Cho, Hong-Yeon | - |
dc.contributor.author | Lee, Heung-Shick | - |
dc.contributor.author | Kim, Young-Wan | - |
dc.date.accessioned | 2021-09-08T04:02:20Z | - |
dc.date.available | 2021-09-08T04:02:20Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-04 | - |
dc.identifier.issn | 1226-7708 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/116669 | - |
dc.description.abstract | Trehalose synthase (TreS) is an enzyme which produces trehalose from maltose through intramolecular transglycosylation. In this study, a gene (cg2529) encoding for TreS from Corynebacterium glutamicum (CgTS) was cloned and expressed in Escherichia coli. The hexahistidinetagged CgTS showed an optimum temperature and pH of 35A degrees C and pH 7.0, respectively. This enzyme was not thermostable, but stable in a broad pH range from pH 5.0 to 8.5. Its activity slightly increased by 5 mM Mg2+ and Fe2+, while it was strongly inhibited by 5 mM sodium dodecyl sulfate (SDS). CgTS catalyzed the conversion from maltose into trehalose, and vice versa. Lowering reaction temperature by 5A degrees C from the optimum temperature significantly reduced hydrolysis activity to produce glucose as a by-product compared to transglycosylation activity to produce trehalose, leading to increase in the conversion yields from maltose into trehalose. Consequently, the maximum conversion yield by CgTS reached 69% at 25A degrees C after 9 hr of reaction. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | EXPRESSION | - |
dc.subject | EVOLUTION | - |
dc.title | Gene cloning and characterization of a trehalose synthase from Corynebacterium glutamicum ATCC13032 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Heung-Shick | - |
dc.contributor.affiliatedAuthor | Kim, Young-Wan | - |
dc.identifier.doi | 10.1007/s10068-010-0079-x | - |
dc.identifier.scopusid | 2-s2.0-79957666610 | - |
dc.identifier.wosid | 000277239600039 | - |
dc.identifier.bibliographicCitation | FOOD SCIENCE AND BIOTECHNOLOGY, v.19, no.2, pp.565 - 569 | - |
dc.relation.isPartOf | FOOD SCIENCE AND BIOTECHNOLOGY | - |
dc.citation.title | FOOD SCIENCE AND BIOTECHNOLOGY | - |
dc.citation.volume | 19 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 565 | - |
dc.citation.endPage | 569 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001441122 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordAuthor | trehalose | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | trehalose synthase | - |
dc.subject.keywordAuthor | intramolecular transglycosylation | - |
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