Anti-diabetic activities of Kocat-D1 in 3T3-L1 adipocytes and C57BL/KsJ-db/db mice
- Authors
- Yang, J.-H.; Won, H.-J.; Park, H.-Y.; Nam, M.-H.; Lee, H.-S.; Lee, J.; Kim, J.-T.; Lee, K.-W.
- Issue Date
- 2010
- Keywords
- 3T3-L1; Anti-diabetes; C57BL/KsJ-db/db mice; Insulin; Kocat-D1
- Citation
- Journal of the Korean Society of Food Science and Nutrition, v.39, no.5, pp.692 - 698
- Indexed
- SCOPUS
KCI
- Journal Title
- Journal of the Korean Society of Food Science and Nutrition
- Volume
- 39
- Number
- 5
- Start Page
- 692
- End Page
- 698
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/118243
- DOI
- 10.3746/jkfn.2010.39.5.692
- ISSN
- 1226-3311
- Abstract
- This study investigated anti-diabetic activity of Kocat-D1, which is a currently used traditional medicine for treatment of diabetes in Shandong, China. Insulin sensitizing activity was observed in a cell-based glucose uptake assay using 3T3-L1 adipocytes. The treatment of 0.2 mg/mL of hot water extract of Kocat-D1 with 0.2 nM insulin was associated with a significant increasing in glucose uptake (165.0±0.7%) over the treatment of 0.2 nM insulin. C57BL/KsJ-db/db mice (8 weeks of age) were separated into 3 groups: normal control (control, db/ mice untreated), diabetic control (DM control, db/db mice untreated), Kocat-D1 (db/db mice treated with Kocat-D1 extract 350 mg/kg/day). After 16 weeks of treatment, body weight and total diet intake of Kocat-D1 group were significantly lower than DM control groups. Blood glucose levels of the Kocat-D1 group (14.7±1.4 mmol/L) were significantly lower compared to the DM control group (27.1±0.2 mmol/L). Furthermore, insulin level was significantly increased in the Kocat-D1 group (0.17±0.02 ng/mL) compared with the DM control group (0.05±0.02 ng/mL). The glomeruli in kidney was stained using periodic acid-shiff base (PAS) for confirming collagen accumulation. The glomeruli in kidney of Kocat-D1 group had significantly reduced PAS-positive compared with that of DM control.
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