Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Synthesis of structured phosphatidylcholine containing n-3 PUFA residues via acidolysis mediated by immobilized phospholipase A1

Authors
Kim, I.-H.Garcia, H.S.Hill, Jr. C.G.
Issue Date
2010
Keywords
Fish oil; Lysophosphatidylcholine; Phosphatidylcholine; Phospholipase A1; Water activity
Citation
JAOCS, Journal of the American Oil Chemists' Society, v.87, no.11, pp.1293 - 1299
Indexed
SCIE
SCOPUS
Journal Title
JAOCS, Journal of the American Oil Chemists' Society
Volume
87
Number
11
Start Page
1293
End Page
1299
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/118308
DOI
10.1007/s11746-010-1609-7
ISSN
0003-021X
Abstract
Structured phosphatidylcholine (PC) was successfully synthesized by acidolysis of PC derived from soybean with n-3 polyunsaturated fatty acids (PUFA) obtained from fish oil using an immobilized phospholipase-A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effect of water activity in the range from 0.43 to 0.93 on n-3 PUFA residue content was investigated. The immobilized phospholipase was more active at water activities greater than 0.53. The yield of PC decreased as the water activity increased from 0.43 to 0.93. The yield of lysophosphatidylcholine (LPC) increased significantly during the first 4 h of reaction as a consequence of hydrolysis of the PC. As the enzyme loading increased, the proportion of n-3 PUFA residues in the PC and LPC present at various times in the mixture of these species increased to as much as 15% of the total weight of substrate. For all enzyme loadings tested, both the proportion of n-3 PUFA residues in the total PC (unreacted and modified) and the production of LPC increased significantly when the reaction time was increased, although differences in n-3 PUFA content were observed. When both the n-3 PUFA content of the PC and the total yield of PC are considered, the optimum water activity and enzyme loading for production of structured PC and LPC are 0.65 and 15%, respectively. © 2010 AOCS.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, In Hwan photo

Kim, In Hwan
College of Health Sciences (School of Biosystems and Biomedical Sciences)
Read more

Altmetrics

Total Views & Downloads

BROWSE