Effects of protein kinase C delta and phospholipase C-gamma 1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death
- Authors
- Kim, Yoon Suk; An, Hyoung Tae; Kim, Jeonghan; Ko, Jesang
- Issue Date
- 12월-2009
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- MCP-1; taxol; protein kinase C delta; phospholipase C; signal transduction; breast cancer
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.24, no.6, pp.853 - 858
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
- Volume
- 24
- Number
- 6
- Start Page
- 853
- End Page
- 858
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/118803
- DOI
- 10.3892/ijmm_00000303
- ISSN
- 1107-3756
- Abstract
- Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that plays an important role in immune cell migration. It has been reported that chemokines, including MCP-1, are involved in angiogenesis and metastasis. However, the exact role of chemokines in cancer development is still obscure. We investigated the involvement of MCP-1 in taxol-induced breast cancer cell death. The anti-cancer drug taxol induced MCF-7 breast cancer cell death. Treatment with taxol increased the mRNA expression level of MCP-1 in it dose- and time-dependent manner. Up-regulation of MCP-1 by taxol was augmented in cells treated with rottlerin, a specific inhibitor of protein kinase C delta (PKC delta). In addition, taxol-induced MCP-1 expression was reduced by the ectopic expression of PKC delta in a dose-dependent manner, indicating that PKC delta plays a negative role in taxol-induced MCP-1 expression in MCF-7 cells. On the other hand, taxol-induced up-regulation of MCP-1 was reduced in cells treated with U73122, an inhibitor of phospholipase C (PLC), and ectopic expression of PLC-gamma 1 increased the expression of MCP-1 in taxol-treated MCF-7 cells, indicating that PLC-gamma 1 functions as a positive regulator in taxol-induced MCP-1 expression. These results indicate that MCP-1 is involved in taxol-induced breast cancer cell death and we propose that taxol induces up-regulation of MCP-1 by affecting both positive and negative regulatory signaling pathways.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.