Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Droplet-based magnetically activated cell separation: analysis of separation efficiency based on the variation of flow-induced circulation in a pendent drop

Authors
Kim, YounghoLee, Sang HoKim, Byungkyu
Issue Date
12월-2009
Publisher
SPRINGER HEIDELBERG
Keywords
Cell separation; Droplet-based magnetically activated cell separation; Flow-induced circulation; Pendent drop; Magnet
Citation
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.395, no.7, pp.2415 - 2421
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume
395
Number
7
Start Page
2415
End Page
2421
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/118851
DOI
10.1007/s00216-009-3131-z
ISSN
1618-2642
Abstract
Under the assumption that separation efficiencies are mainly affected by the velocity of flow-induced circulation due to buffer injection in a pendent drop, this paper describes an analysis of the separation efficiency of a droplet-based magnetically activated cell separation (DMACS) system. To investigate the velocity of the flow-induced circulation, we supposed that numerous flows in a pendent drop could be considered as a "theoretically normalized" flow (or conceptually normalized flow, CNF) based on the Cauchy-Goursat theorem. With the morphological characteristics (length and duration time) of a pendent drop depending on the initial volume, we obtained the velocities of the CNF. By measuring the separation efficiencies for different initial volumes and by analyzing the separation efficiency in terms of the velocity of the CNF, we found that the separation efficiencies (in the case of a low rate of buffer injection; 5 and 15 mu l center dot min(-1)) are mainly affected by the velocity of the CNF. Moreover, we confirmed that the phenomenological features of a pendent drop cause a fluctuation of its separation efficiencies over a range of specific volumes (initial volumes ranging from 40 to 80 A mu l), because of the "sweeping-off" phenomenon, that is, positive cells gathered into the positive fraction are forced to move away from the magnetic side by flow-induced circulation due to buffer injection. In addition, from the variation of the duration time, that is, the interval between the beginning of injection of the buffer solution and the time at which a pendent drop detaches, it could also be confirmed that a shorter duration time leads to decrease of the number of positive cells in negative fraction regardless of the rate of buffer injection (5, 15, and 50 mu l center dot min(-1)). Therefore, if a DMACS system is operated with a 15 mu l center dot min(-1) buffer injection flow rate and an initial volume of 80 mu l or more, we would have the best efficiency of separation in the negative fraction.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE