Absolute CD4(+) Cell Count Using a Plastic Microchip and a Microscopic Cell Counter
- Authors
- Bae, Sook Young; Park, Hae Chul; Oh, Jun Seo; Yoon, Soo-Young; Park, Dae Won; Choi, In Keun; Kim, Hae Joong; Oh, Jong Hyun; Hur, Dae Sung; Chung, Chanil; Chang, Jun Keun; Robinson, J. Paul; Lim, Chae Seung
- Issue Date
- 9월-2009
- Publisher
- WILEY
- Keywords
- antigens; CD4; CD4 lymphocyte count; flow cytometry; microchip; absolute CD4 count; microscopic cell counter
- Citation
- CYTOMETRY PART B-CLINICAL CYTOMETRY, v.76B, no.5, pp.345 - 353
- Indexed
- SCIE
SCOPUS
- Journal Title
- CYTOMETRY PART B-CLINICAL CYTOMETRY
- Volume
- 76B
- Number
- 5
- Start Page
- 345
- End Page
- 353
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/119356
- DOI
- 10.1002/cyto.b.20470
- ISSN
- 1552-4949
- Abstract
- We have designed and evaluated the performance of a simple, rapid, and affordable method for counting CD4(+) T-cells with the use of plastic microchips. This new system is an adaptation of a "no-lyse, no-wash," volumetric single platform assay, and absolute CD4(+) counts are determined with the use of a microscopic scanning cell counter. To assess the CD4(+) count test precision and linearity of the system, measured CD4(+) counts were compared with two other reference assays (single and dual platform flow cytometry) with the use of 123 clinical samples including samples obtained from 35 HIV-infected patients, and artificially diluted samples. A correlation between the results from the use of the new method and from the use of the two other reference assays was r = 0.98 for the clinical samples. A dilution test of the new method demonstrated a linearity of r >= 0.99, with coefficients of variation <= 7.6% for all concentration levels. Our findings suggest that the new CD4(+) counting device can be potentially be applied for other diagnostic procedures that measure quantities of characteristic antigens or other materials on cells. (C) 2009 Clinical Cytometry Society
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- Appears in
Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
- College of Medicine > Department of Medical Science > 1. Journal Articles
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