Comparison of the Novel Human Papillomavirus 4 Auto-capillary Electrophoresis Test with the Hybrid Capture 2 Assay and with the PCR HPV Typing Set Test in the Detection of High-Risk HPV Including HPV 16 and 18 Genotypes in Cervical Specimens
- Authors
- Hong, Jin Hwa; Song, Seung Hun; Kim, Jong Kee; Han, Jeong Hyun; Lee, Jae Kwan
- Issue Date
- 8월-2009
- Publisher
- KOREAN ACAD MEDICAL SCIENCES
- Keywords
- HPV Typing Methods; ACE; HC2; HPV Typing Set Test
- Citation
- JOURNAL OF KOREAN MEDICAL SCIENCE, v.24, no.4, pp.579 - 584
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF KOREAN MEDICAL SCIENCE
- Volume
- 24
- Number
- 4
- Start Page
- 579
- End Page
- 584
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/119591
- DOI
- 10.3346/jkms.2009.24.4.579
- ISSN
- 1011-8934
- Abstract
- The aim of this study was to compare the novel human papillomavirus (HPV) detection method, the HPV 4 Auto-capillary Electrophoresis (ACE) test with the hybrid capture (HC) 2 assay for the detection of high-risk HPVs. In addition, we compared the HPV 4 ACE test with the polymerase chain reaction HPV Typing Set test for the detection of HPV 16 and HPV 18 genotypes. One hundred ninety-nine cervical swab samples obtained from women with previous abnormal Pap smears were subjected to testing with the three HPV tests. The HPV 4 ACE test and the HC 2 assay showed substantial agreement for detection of high-risk HPVs (85.4%, kappa=0.71). The HPV 4 ACE test also showed substantial agreement with the PCR HPV Typing Set test in the detection of HPV 16 and HP V 18 genotypes (89.9%, kappa=0.65). In correlation with cytologic results, the sensitivities and specificities of the HPV 4 ACE test and HC 2 assay were 92.9% vs. 92.9% and 48.1% vs. 50.8%, respectively, when high-grade squamous intraepithelial lesions were regarded as abnormal cytologies. The novel HPV 4 ACE test is a valuable tool for the detection of high-risk HPVs and for genotyping of HPV 16 and HPV 18.
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