Enzymatic Preparation of Maltooctaose-rich Mixture from Starch Using a Debranching Enzyme of Nostoc punctiforme
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Ji-Hye | - |
dc.contributor.author | Kim, Myo-Jeong | - |
dc.contributor.author | Kim, Young-Wan | - |
dc.contributor.author | Lee, Heeseob | - |
dc.contributor.author | Park, Jong-Tae | - |
dc.contributor.author | Lee, Byong-Hoon | - |
dc.contributor.author | Park, Kwan-Hwa | - |
dc.date.accessioned | 2021-09-08T18:27:51Z | - |
dc.date.available | 2021-09-08T18:27:51Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.issn | 1226-7708 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120331 | - |
dc.description.abstract | The debranching enzyme of Nostoc punctiforme (NPDE) is a novel enzyme that catalyzes the hydrolysis of alpha-1,6-glycosidic linkages in starch, followed by the sequential hydrolysis of alpha-1,4-glycosidic linkages. The debranching activity of NPDE is highly specific for branched chains with a degree of polymerization (DP)>8. Moreover, the rate of hydrolysis of alpha-1,4-linkages by NPDE is greatly enhanced for maltooligosaccharides (MoS) With a DP>8. An analysis of reaction mixtures containing various starches revealed the accumulation of maltooctaose (G8) with glucose and maltose. Based on the novel enzymatic properties of NPDE, an MO mixture containing more than 60% G8 with yield of 18 g G8 for 100 g starch was prepared by the reaction of NPDE with soluble starch, followed by ethanol precipitation and gel permeation chromatography (GPC). The yield of the G8-rich mixture was significantly improved by the addition of isoamylase. In summary, a 4-step process of the production of a G8-rich mixture was developed involving the enzymatic hydrolysis of starch by NPDE. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST | - |
dc.subject | ALPHA-AMYLASE | - |
dc.subject | MALTOHEXAOSE | - |
dc.title | Enzymatic Preparation of Maltooctaose-rich Mixture from Starch Using a Debranching Enzyme of Nostoc punctiforme | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Wan | - |
dc.identifier.scopusid | 2-s2.0-77955095424 | - |
dc.identifier.wosid | 000265748900049 | - |
dc.identifier.bibliographicCitation | FOOD SCIENCE AND BIOTECHNOLOGY, v.18, no.2, pp.570 - 573 | - |
dc.relation.isPartOf | FOOD SCIENCE AND BIOTECHNOLOGY | - |
dc.citation.title | FOOD SCIENCE AND BIOTECHNOLOGY | - |
dc.citation.volume | 18 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 570 | - |
dc.citation.endPage | 573 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001339161 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | ALPHA-AMYLASE | - |
dc.subject.keywordPlus | MALTOHEXAOSE | - |
dc.subject.keywordAuthor | Nostoc punctiforme | - |
dc.subject.keywordAuthor | cyanobacteria | - |
dc.subject.keywordAuthor | debranching enzyme | - |
dc.subject.keywordAuthor | maltooctaose-rich mixture | - |
dc.subject.keywordAuthor | maltooligosaccharide | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.