Antipathogenic Properties of Green Tea Polyphenol Epigallocatechin Gallate at Concentrations below the MIC against Enterohemorrhagic Escherichia coli O157:H7
- Authors
- Lee, Kang-Mu; Kim, Wan-Seok; Lim, Jeesun; Nam, Sunyoung; Youn, Min; Nam, Seong-Won; Kim, Younghoon; Kim, Sae-Hun; Park, Woojun; Park, Sungsu
- Issue Date
- Feb-2009
- Publisher
- INT ASSOC FOOD PROTECTION
- Citation
- JOURNAL OF FOOD PROTECTION, v.72, no.2, pp.325 - 331
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF FOOD PROTECTION
- Volume
- 72
- Number
- 2
- Start Page
- 325
- End Page
- 331
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120674
- DOI
- 10.4315/0362-028X-72.2.325
- ISSN
- 0362-028X
- Abstract
- The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O15TH7 were demonstrated at concentrations of 1 to 100 mu g/ml, which are lower than the MIC (539 +/- 22 mu g/ml). At 25 mu g/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 mu g/ml. Our results suggest that EGCG at concentrations below its MIC has significant antipathogenic effects against E. coli O157:H7.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
- College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles
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