Chemical exchange of hydroxyl protons in quercetin measured by pulsed field gradient NMR
- Authors
- Hong, Y. -S.; Hong, K. S.; Cho, J. -H.; Volkov, V. I.; Lee, C. -H.
- Issue Date
- 2월-2009
- Publisher
- SPRINGER WIEN
- Citation
- APPLIED MAGNETIC RESONANCE, v.35, no.2, pp.261 - 270
- Indexed
- SCIE
SCOPUS
- Journal Title
- APPLIED MAGNETIC RESONANCE
- Volume
- 35
- Number
- 2
- Start Page
- 261
- End Page
- 270
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120687
- DOI
- 10.1007/s00723-008-0160-2
- ISSN
- 0937-9347
- Abstract
- The diffusion behavior of hydroxyl protons (OH) in quercetin in 100% DMSO-d(6) (deuterium dimethylsulfoxide) and a 90% DMSO-d(6) solution containing 10% H2O was investigated with 600 MHz H-1 pulsed field gradient (PFG) nuclear magnetic resonance (NMR). Only resonances of the 5-hydroxyl protons (OH5) were well resolved in NMR spectra of quercetin for all solutions under study. This phenomenon is explained by the intramolecular hydrogen bonding between OH5 protons and the 4-carbonyl oxygen (CO4). During diffusion experiments, the OH5 protons showed a biexponential diffusion decay, indicating an exchange process with water. As water content in the solvents increased, the lifetime (tau (q)) of the OH5 protons decreased from 96.7 +/- 10.0 ms in 100% DMSO-d(6) to 14.3 +/- 1.4 ms in the 90% DMSO-d(6) solution containing 10% H2O, indicating an increase in the exchange rate (k (q) = l/tau (q)) of the OH5 protons. This study demonstrates that the diffusion approach with PFG-NMR is much faster and easier for estimating the short lifetime or fast exchange rate of hydroxyl protons in quercetin.
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Collections - College of Life Sciences and Biotechnology > Division of Food Bioscience and Technology > 1. Journal Articles
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