Stabilization of Glycoprotein Liquid Formulation Using Arginine: A Study with Lactoferrin as a Model Protein
- Authors
- Kim, Hyun-Jung; Shin, Chang hoon; Kim, Chan-Wha
- Issue Date
- Jan-2009
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- liquid formulation; human serum albumin; arginine; lactoferrin; stabilizing agent
- Citation
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.73, no.1, pp.61 - 66
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- Volume
- 73
- Number
- 1
- Start Page
- 61
- End Page
- 66
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120817
- DOI
- 10.1271/bbb.80475
- ISSN
- 0916-8451
- Abstract
- The formulation of new biotherapeutics without human serum albumin (HSA) could decrease the potential risk of blood-transmitted diseases and those caused by infectious viruses and other pathogens. In the present study, arginine was examined as a potential alternative to HAS, and bovine lactoferrin (bLf) was used as a representative model glycoprotein since bLf has potential immunomodulatory and antiviral activity. The optimal formulation for the mixture was determined to be 10 mM arginine, 15% (w/v) trehalose, and 0.02% (v/v) Tween 80, using a statistical analysis program, Minitab. Analyses were performed using reverse-phase high-performance liquid chromatography (HPLC) and SDS-PAGE. The blf HSA-free formulations lost only 12-20% of blf compared with 46% for control (without additives) after 28d of storage. Based on long-term stability studies, the HSA-free formulation developed in this study had a stronger effect on the stability of bLf (1.4-fold) than HSA formulation tinder various storage conditions over 6 months.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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