Determination of tocopherol contents in refined edible oils using an HPLC method
- Authors
- Hu, J.-N.; Zhu, X.-M.; Adhikari, P.; Li, D.; Kim, I.-H.; Lee, K.-T.
- Issue Date
- 2009
- Keywords
- HPLC separation; Refined edible oils; Tocopherols
- Citation
- Journal of Food Science and Nutrition, v.14, no.3, pp.260 - 264
- Indexed
- SCOPUS
KCI
- Journal Title
- Journal of Food Science and Nutrition
- Volume
- 14
- Number
- 3
- Start Page
- 260
- End Page
- 264
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/121873
- DOI
- 10.3746/jfn.2009.14.3.260
- ISSN
- 1226-332X
- Abstract
- A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression (r 2>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94∼4.27 and 1.77∼4.88%, respectively. The tocopherol recoveries ranged from 91.44∼108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of α, γ, and δ-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.