Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability
- Authors
- Kim, Choong; Lee, Kang Sun; Kim, Young Eun; Lee, Kyu-Jung; Lee, Soo Hyun; Kim, Tae Song; Kang, Ji Yoon
- Issue Date
- 2009
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- LAB ON A CHIP, v.9, no.9, pp.1294 - 1297
- Indexed
- SCIE
SCOPUS
- Journal Title
- LAB ON A CHIP
- Volume
- 9
- Number
- 9
- Start Page
- 1294
- End Page
- 1297
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122114
- DOI
- 10.1039/b819044e
- ISSN
- 1473-0197
- Abstract
- This paper describes a microfluidic device for the microencapsulation of cells in alginate beads to enhance cell viability. The alginate droplet including cells was gelified with calcified oleic acid, using two-phase microfluidics. The on-chip gelation had generated monodisperse spherical alginate beads, which could not be readily obtained via conventional external gelation in a calcium chloride bath. However, the prolonged exposure of encapsulated cells to the toxic oil phase caused serious damage to the cells. Therefore, we proposed the formulation of a rapid oil-exchange chip which transforms the toxic oleic acid to harmless mineral oil. The flushing out of oleic acid after the gelation of alginate beads effected a dramatic increase in the viability of P19 embryonic carcinoma cells, up to 90%. The experimental results demonstrated that the cell viability was proportional to the flow rate of squeezing mineral oil.
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Collections - Graduate School > Graduate School of management of technology > 1. Journal Articles
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