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Improved serum stability and biophysical properties of siRNAs following chemical modifications
- Authors
- Cho, Ik Sang; Kim, Jung; Lim, Do Hwan; Ahn, Hee-Chul; Kim, Hyunggee; Lee, Kang-Bong; Lee, Young Sik
- Issue Date
- Nov-2008
- Publisher
- SPRINGER
- Keywords
- A-form helix; melting temperature; 2 '-ribose modification; RNA interference; siRNA; stability
- Citation
- BIOTECHNOLOGY LETTERS, v.30, no.11, pp 1901 - 1908
- Pages
- 8
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY LETTERS
- Volume
- 30
- Number
- 11
- Start Page
- 1901
- End Page
- 1908
- ISSN
- 0141-5492
1573-6776
- Abstract
- Small interfering RNAs (siRNAs) which mediate sequence-specific gene silencing through RNA interference have been harnessed for therapeutic applications. Here, we found combinations of modest 2'-ribose siRNA modifications that permit effective gene silencing and improve siRNA stability in human serum. Introduction of as few as two different or identical 2'-ribose modifications at a specific position of either strand, which has been previously suggested to suppress unintended off-target effects, enhances the thermal and thermodynamic stability of siRNA duplexes, and does not alter the overall A-form helical geometry that is required for silencing activity. Our results provide a useful template for the design for therapeutic siRNAs.
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Related Researcher
- Kim, Hyung gee
- Graduate School (Department of Biotechnology)