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Sample preparation method for plasma membrane proteome analysis
- Issue Date
- 1-Sep-2008
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- hMSCs; Percoll; plasma membrane; two-phase extraction; two-dimensional electrophoresis; Ultracentrifugation
- Citation
- JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.872, no.1-2, pp.177 - 180
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
- Volume
- 872
- Number
- 1-2
- Start Page
- 177
- End Page
- 180
- ISSN
- 1570-0232
- Abstract
- The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis. (c) 2008 Elsevier B.V. All rights reserved.
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