Degradation of cellulose by the major endoglucanase produced from the brown-rot fungus Fomitopsis pinicola
- Authors
- Yoon, Jeong-Jun; Cha, Chang-Jun; Kim, Yeong-Suk; Kim, Wook
- Issue Date
- Aug-2008
- Publisher
- SPRINGER
- Keywords
- brown-rot fungus; cellulose; endoglucanase; Fomitopsis pinicola; microcrystalline cellulose hydrolysis
- Citation
- BIOTECHNOLOGY LETTERS, v.30, no.8, pp.1373 - 1378
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY LETTERS
- Volume
- 30
- Number
- 8
- Start Page
- 1373
- End Page
- 1378
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122916
- DOI
- 10.1007/s10529-008-9715-4
- ISSN
- 0141-5492
- Abstract
- An endoglucanase that is able to degrade both crystalline and amorphous cellulose was purified from the culture filtrates of the brown-rot fungus Fomitopsis pinicola grown on cellulose. An apparent molecular weight of the purified enzyme was similar to 32 kDa by SDS-PAGE analysis. The enzyme was purified 11-fold with a specific activity of 944 U/mg protein against CMC. The partial amino acid sequences of the purified endoglucanase had high homology with endo-beta-1,4-glucanase of glycosyl hydrolase family 5 from other fungi. The K-m and K-cat values for CMC were 12 mg CMC/ml and 670/s, respectively. The purified EG hydrolyzed both cellotetraose (G4) and cellopentaose (G5), but did not degrade either cellobiose (G2) or cellotriose (G3).
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Collections - Graduate School > Department of Plant Biotechnology > 1. Journal Articles
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