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Preparation of feather digests as fertilizer with Bacillus pumilis KHS-1

Authors
Kim, JMChoi, YMSuh, HJ
Issue Date
Jun-2005
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Keywords
Bacillus pumilis KHS-1; feather digests; keratinolytic protease; fertilizer
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.15, no.3, pp.472 - 476
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume
15
Number
3
Start Page
472
End Page
476
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/123233
ISSN
1017-7825
Abstract
The present study was untaken to assess the capacity of Bacillus pumilis KHS-1 to grow on chicken flour and to prepare feather digest as fertilizer. To increase keratinolytic activity, the addition of cysteine (5.0 mM) showed the highest keratinolytic activity (245 unit) among the reducing agents tested. The production of soluble protein (feather digests) paralleled the tendency to the production of keratinolytic protease. In the growth curve of B. pumilis KHS-1 at 30 degrees C in the feather medium with 5 mM cysteine, the maximum keratinolytic activity of B. pumilis was about 161 units/ml after 84 It of incubation. The maximum enzyme activities were observed at the late logarithmic growth phase, and remained thereafter with little changes. Using 27-day plant growth assays on carrot and Chinese cabbage, feather digests and reference fertilizer were compared. In terms of the length and the weight of the above-ground vegetations, feather digests showed the same effect as that of the fertilizer. Therefore, our investigation shows that the feather digests can be used in agriculture.
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