Beneficial effect of the unsaponifiable matter from rice bran on oxidative stress in vitro compared with alpha-tocopherol
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, JW | - |
dc.contributor.author | Lee, SW | - |
dc.contributor.author | Kim, MK | - |
dc.contributor.author | Rhee, C | - |
dc.contributor.author | Kim, IH | - |
dc.contributor.author | Lee, KW | - |
dc.date.accessioned | 2021-09-09T06:56:10Z | - |
dc.date.available | 2021-09-09T06:56:10Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2005-02 | - |
dc.identifier.issn | 0022-5142 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123260 | - |
dc.description.abstract | Unsaponifiable matter (UM) was prepared from rice bran using n-hexane extraction followed by removal of its fatty acid methyl ester with supercritical CO2 under heat-stable conditions. The UM was made up of 1% of vitamin E isomers, 28% of gamma-oryzanol and 71% of uncharacterized compounds. The aim of this study was to determine the antioxidant activities of the UM, using alpha-tocopherol (alpha-T) as a positive control, by measuring the Fe3+-reducing antioxidant power (FRAP), 2,2-diphenyl-l-picrylhydrazyl (DPPH.) free-radical-scavenging property and lipid peroxidation in rat liver microsomes. In addition, the effects of the UM on the tert-butyl hydroperoxide (t-BOOH)-induced cytotoxicity in cultured rat hepatocytes were also investigated. In FRAP assay and DPPH. free-radical-scavenging assay, the results were expressed g(-1) alpha-T or g(-1) UM. The amount of UM used in lipid peroxidation assay and cytotoxicity assay was the amount required to have equal amounts of total vitamin E isomers in the sample and the control alpha-T. The UM, as well as alpha-T, exhibited significant antioxidant activities in FRAP, radical-scavenging and membrane-lipid oxidation. The FRAP value for total vitamin E isomers in the UM (TVEIUM) was 9.1 times higher than that for alpha-T. In terms of their capacities to perform radical-scavenging and lipid peroxidation, both TVEIUM and alpha-T showed similar antioxidant activities. In experiments using cultured rat hepatocytes, the t-BOOH-induced lactate dehydrogenase release was significantly inhibited by the addition of 63.5 and 160 mug ml(-1) of TVEIUM treatments (84 and 89%, respectively), and that of 63.5 and 160 mug ml(-1) of alpha-T treatment (88 and 93%, respectively). The antioxidant function against oxidative stress of the UM prepared from rice bran may extend its use to being a potential dietary supplement. (C) 2004 Society of Chemical Industry. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | ADULT RAT-LIVER | - |
dc.subject | CANCER PREVENTION | - |
dc.subject | PARENCHYMAL-CELLS | - |
dc.subject | GAMMA-ORYZANOL | - |
dc.subject | REDOX STATE | - |
dc.subject | OIL | - |
dc.subject | ANTIOXIDANTS | - |
dc.subject | CHOLESTEROL | - |
dc.subject | TOCOTRIENOLS | - |
dc.subject | GLUTATHIONE | - |
dc.title | Beneficial effect of the unsaponifiable matter from rice bran on oxidative stress in vitro compared with alpha-tocopherol | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Rhee, C | - |
dc.contributor.affiliatedAuthor | Kim, IH | - |
dc.contributor.affiliatedAuthor | Lee, KW | - |
dc.identifier.doi | 10.1002/jsfa.2009 | - |
dc.identifier.scopusid | 2-s2.0-13444291735 | - |
dc.identifier.wosid | 000226590300020 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, v.85, no.3, pp.493 - 498 | - |
dc.relation.isPartOf | JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | - |
dc.citation.title | JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | - |
dc.citation.volume | 85 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 493 | - |
dc.citation.endPage | 498 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | ADULT RAT-LIVER | - |
dc.subject.keywordPlus | CANCER PREVENTION | - |
dc.subject.keywordPlus | PARENCHYMAL-CELLS | - |
dc.subject.keywordPlus | GAMMA-ORYZANOL | - |
dc.subject.keywordPlus | REDOX STATE | - |
dc.subject.keywordPlus | OIL | - |
dc.subject.keywordPlus | ANTIOXIDANTS | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordPlus | TOCOTRIENOLS | - |
dc.subject.keywordPlus | GLUTATHIONE | - |
dc.subject.keywordAuthor | gamma-oryzanol | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | rice bran | - |
dc.subject.keywordAuthor | supercritical CO2 | - |
dc.subject.keywordAuthor | tocopherol | - |
dc.subject.keywordAuthor | unsaponifiable matter | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.