PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells
- Authors
- Jang, Chan Ho; Lee, In Ae; Ha, Young Ran; Lim, Jinkyu; Sung, Mi-Kyung; Lee, Sung-Joon; Kim, Jong-Sang
- Issue Date
- Jul-2008
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- phosphoglycerate kinase 1 (PGK1); oxidative stress; antioxidant; biomarker
- Citation
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.72, no.7, pp.1799 - 1808
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- Volume
- 72
- Number
- 7
- Start Page
- 1799
- End Page
- 1808
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/123291
- DOI
- 10.1271/bbb.80079
- ISSN
- 0916-8451
- Abstract
- Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including alpha-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.