Enzymatic synthesis of dimaltosyl-beta-cyclodextrin via a transglycosylation reaction using TreX, a Sulfolobus solfataricus P2 debranching enzyme
DC Field | Value | Language |
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dc.contributor.author | Kang, Hee-Kwon | - |
dc.contributor.author | Cha, Hyunju | - |
dc.contributor.author | Yang, Tae-Joo | - |
dc.contributor.author | Park, Jong-Tae | - |
dc.contributor.author | Lee, Seungjae | - |
dc.contributor.author | Kim, Young-Wan | - |
dc.contributor.author | Auh, Joong-Hyuck | - |
dc.contributor.author | Okada, Yasuyo | - |
dc.contributor.author | Kim, Jung-Wan | - |
dc.contributor.author | Cha, Jaeho | - |
dc.contributor.author | Kim, Chung Ho | - |
dc.contributor.author | Park, Kwan-Hwa | - |
dc.date.accessioned | 2021-09-09T11:26:13Z | - |
dc.date.available | 2021-09-09T11:26:13Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-02-01 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/124106 | - |
dc.description.abstract | Di-O-alpha-maltosyl-beta-cyclodextrin ((G2)(2)-beta-CD) was synthesized from 6-O-alpha-maltosyl-beta-cyclodextrin (G2-beta-CD) via a transglycosylation reaction catalyzed by TreX, a debranching enzyme from Sulfolobus solfataricus P2. TreX showed no activity toward glucosyl-beta-CD, but a transfer product (1) was detected when the enzyme was incubated with maltosyl-beta-CD, indicating specificity for a branched glucosyl chain bigger than DP2. Analysis of the structure of the transfer product (1) using MALDI-TOF/MS and isoamylase or glucoamylase treatment revealed it to be dimaltosyl-beta-CD, suggesting that TreX transferred the maltosyl residue of a G2-beta-CD to another molecule of G2-beta-CD by forming an alpha-1,6-glucosidic linkage. When [C-14]-maltose and maltosyl-beta-CD were reacted with the enzyme, the radiogram showed no labeled dimaltosyl-beta-CD; no condensation product between the two substrates was detected, indicating that the synthesis of dimaltosyl-beta-CD occurred exclusively via transglycosylation of an alpha-1,6-glucosidic linkage. Based on the HPLC elution profile, the transfer product (1) was identified to be isomers of 6(1),6(3) - and 6(1),6(4) -dimaltosyl-beta-CD. Inhibition studies with P-CD on the transglycosylation activity revealed that beta-CD was a mixed-type inhibitor, with a K-i value of 55.6 mu mol/mL. Thus, dimaltosyl-beta-CD can be more efficiently synthesized by a transglycosylation reaction with TreX in the absence of beta-CD. Our findings suggest that the high yield of (G2)(2)-beta-CD from G2-beta-CD was based on both the transglycosylation action mode and elimination of the inhibitory effect of beta-CD. (C) 2007 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | PSEUDOMONAS ISOAMYLASE | - |
dc.subject | REVERSE ACTION | - |
dc.subject | PULLULANASE | - |
dc.title | Enzymatic synthesis of dimaltosyl-beta-cyclodextrin via a transglycosylation reaction using TreX, a Sulfolobus solfataricus P2 debranching enzyme | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Wan | - |
dc.identifier.doi | 10.1016/j.bbrc.2007.11.115 | - |
dc.identifier.scopusid | 2-s2.0-37549014590 | - |
dc.identifier.wosid | 000252392400016 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.366, no.1, pp.98 - 103 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 366 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 98 | - |
dc.citation.endPage | 103 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | PSEUDOMONAS ISOAMYLASE | - |
dc.subject.keywordPlus | REVERSE ACTION | - |
dc.subject.keywordPlus | PULLULANASE | - |
dc.subject.keywordAuthor | Sulfolobus solfataricus P2 glycogen-debranching-like enzyme (TreX) | - |
dc.subject.keywordAuthor | alpha-(1,6)-Transglycosylation activity | - |
dc.subject.keywordAuthor | dimaltosyl-beta-cyclodextrin ((G2)(2)-beta-CD) | - |
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