Effect of Treadmill Training on ERK-Mediated Schwann Cell Proliferation and Axonal Regrowth in the Regenerating Sciatic NervesEffect of Treadmill Training on ERK-Mediated Schwann Cell Proliferation and Axonal Regrowth in the Regenerating Sciatic Nerves
- Other Titles
- Effect of Treadmill Training on ERK-Mediated Schwann Cell Proliferation and Axonal Regrowth in the Regenerating Sciatic Nerves
- Authors
- Tae-Beom Seo; Myung-Jin Oh; 윤진환; 윤성진; 박해찬; Kyung-Hee Kang; 남궁욱
- Issue Date
- 2008
- Publisher
- 한국체육학회
- Keywords
- Sciatic nerve; ERK; Schwann cell; proliferation; treadmill training; phosphorylation; PD98059
- Citation
- INTERNATIONAL JOURNAL OF HUMAN MOVEMENT SCIENCE, v.2, no.1, pp.63 - 77
- Journal Title
- INTERNATIONAL JOURNAL OF HUMAN MOVEMENT SCIENCE
- Volume
- 2
- Number
- 1
- Start Page
- 63
- End Page
- 77
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/124860
- ISSN
- 1976-4391
- Abstract
- Proliferation of Schwann cells present in distal stump of injured sciatic nerve is a critical step for successful regeneration and this process is facilitated by physical activity. Extracellular signal-regulated kinase 1/2 (ERK1/2) regulates proliferation and differentiation of non-neuronal cells, but correlation between ERK1/2 activation and exercise remains mostly unknown. Here we show that phosphorylation of ERK1/2 (p-ERK1/2) mediates proliferation of Schwann cells at early stage after injury and treadmill training (TMT) is a key regulator for upreulation of p-ERK1/2 induction levels via activation of Schwann cells. Using primary cell culture techniques, we identified the positive effect of low- intensity TMT on neurite outgrowth of dorsal root ganglions (DRG) and proliferation of Schwann cells in normal rat. After sciatic nerve injury, TMT increased induction levels of GAP-43 protein, regeneration marker, according to the passage of TMT duration and sustained phosphorylation of ERK1/2 by 7 days after injury. Also transcription factor c-Jun related with MAPK was observed in injured nerve at 3 days post crush and largely upregulated by daily TMT performance. p-ERK1/2 protein expressed in Schwann cells was distally transported and accelerated by TMT at 1 and 3 days post crush. We further demonstrate that TMT promoted axonal regeneration 3 days after sciatic nerve injury, greatly suppressed by in vivo administration of ERK kinase inhibitor PD98059. Thus, the present data provide a new evidence that TMT-mediated enhancement of axonal regeneration involves dynamic regulation of Schwann cell activity by phosphorylation of ERK1/2.
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Collections - College of Education > Department of Physical Education > 1. Journal Articles
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