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Production of anti-complementary exopolysaccharides from submerged culture of Flammulina velutipes

Authors
Shin, Kwang-SoonYu, Kwang-WonLee, Hyun-KyungLee, HoCho, Won-DaiSuh, Hyung-Joo
Issue Date
Jul-2007
Publisher
FACULTY FOOD TECHNOLOGY BIOTECHNOLOGY
Keywords
anti-complementary; exopolysaccharides; submerged culture; bioreactor; Flammulina velutipes
Citation
FOOD TECHNOLOGY AND BIOTECHNOLOGY, v.45, no.3, pp.319 - 326
Indexed
SCIE
SCOPUS
Journal Title
FOOD TECHNOLOGY AND BIOTECHNOLOGY
Volume
45
Number
3
Start Page
319
End Page
326
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/125750
ISSN
1330-9862
Abstract
Seven species of basidiomycetes have been investigated for anti-complementary activity in hot water extracts and ethanol soluble fractions. Since Flammulina velutipes had the most potent activity, culture conditions for its mycelial growth were optimized to increase the production efficiency of anti-complementary exopolysaccharides. The optimal medium composition was (in g/L): galactose 15, sodium nitrate 5, glutamic acid 3, KH2PO4 2.5 and MgSO4 center dot 7H(2)O 0.6. Optimal production of anti-complementary activity was achieved at PH=3.5-5.5 and 25 degrees C. With these optimal medium and culture conditions, mycelial dry mass was maximized at 3.17 mg/mL after 6 days of cultivation in a 5-liter stirred-tank bioreactor, without pH control. The anti-complementary activity of exopolysaccharides increased sharply after 4 days of cultivation, and showed a high level at 5-6 days of cultivation. A periodate-labile carbohydrate moiety played a leading role in the anti-complementary activity exhibited by exopolysaccharide produced 2 from F velutipes. Results of tests on the anti-complementary activity in the absence of Ca2+ and immunoelectrophoresis indicated that the mode of complement activation by exopolysaccharide from F velutipes is via both the classical and alternative pathways and that the activation degree is almost the same in each pathway.
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