Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Phospholipase A(1)-catalyzed synthesis of phospholipids enriched in n-3 polyunsaturated fatty acid residues

Authors
Kim, In-HwanGarcia, Hugo S.Hill, Charles Graham, Jr.
Issue Date
3-Apr-2007
Publisher
ELSEVIER SCIENCE INC
Keywords
acidolysis; fish oil; phosphatidylcholine; phospholipase A(1); n-3 polyunsaturated fatty acid (PUFA)
Citation
ENZYME AND MICROBIAL TECHNOLOGY, v.40, no.5, pp.1130 - 1135
Indexed
SCIE
SCOPUS
Journal Title
ENZYME AND MICROBIAL TECHNOLOGY
Volume
40
Number
5
Start Page
1130
End Page
1135
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/125787
DOI
10.1016/j.enzmictec.2006.08.018
ISSN
0141-0229
Abstract
Phosphatidylcholine (PC) was successfully modified by phospholipase A(1)-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A, from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6h of reaction at 55 degrees C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PLTFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC. (c) 2006 Elsevier Inc. All rights reserved.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, In Hwan photo

Kim, In Hwan
College of Health Sciences (School of Biosystems and Biomedical Sciences)
Read more

Altmetrics

Total Views & Downloads

BROWSE