Generation of a WA14 hESC sub-line carrying a hemizygous ABCD1 (C.1696_1710 del) mutation introduced by CRISPR/Cas9 technology
- Authors
- Hong, W.; Yun, W.; Choi, W.; Son, D.; Song, G.; You, S.
- Issue Date
- Apr-2021
- Publisher
- Elsevier B.V.
- Citation
- Stem Cell Research, v.52
- Indexed
- SCIE
SCOPUS
- Journal Title
- Stem Cell Research
- Volume
- 52
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/128999
- DOI
- 10.1016/j.scr.2021.102244
- ISSN
- 1873-5061
1876-7753
- Abstract
- ATP-binding cassette transporter subfamily D member 1 (ABCD1) gene is a member of ABC transporter super family, which conduct peroxisomal import of very long chain fatty acid and crucial underlying factor that induces X-linked adrenoleukodystrophy (X-ALD) when the gene is defected. Here, we report the generation of a human embryonic stem cell sub-line harboring a hemizygous ABCD1 mutation (C.1696_1710 del) using CRISPR/Cas9 system. Established line expresses pluripotency marker genes, can be differentiated to three germ layers, and maintains a normal karyotype. © 2021 The Authors
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