Performance Evaluation of Alere i Influenza A&B in Detecting Influenza Viruses A and B
- Authors
- Kim, Ha Nui; Jang, Woong Sik; Nam, Jeonghun; Mihn, Do-Cic; Lim, Chae Seung
- Issue Date
- 1월-2021
- Publisher
- ASSOC CLINICAL SCIENTISTS
- Keywords
- influenza; Alere i Influenza A& B; RIDT; Real-time PCR
- Citation
- ANNALS OF CLINICAL AND LABORATORY SCIENCE, v.51, no.1, pp.106 - 111
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANNALS OF CLINICAL AND LABORATORY SCIENCE
- Volume
- 51
- Number
- 1
- Start Page
- 106
- End Page
- 111
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/129448
- ISSN
- 0091-7370
- Abstract
- Objective. Alere i Influenza A&B is an isothermal nucleic acid amplification-based integrated system used for detecting and differentiating between influenza virus A and influenza virus 13. We evaluated the clinical performances of Alere i Influenza A&B compared to that of real-time PCR, multiplex real-time PCR, and two rapid influenza diagnostic kits. Methods. Nasopharyngeal aspiration specimens (n=315) from patients with signs of acute respiratory infection were collected between 2015 and 2016. Samples were tested using real-time PCR, the multiplex RT-PCR Anyplex II RV16 Detection kit, Akre i Influenza A&B, BD Veritor (TM) System Flu A+B, and the Sofia Influenza A+B Fluorescence Immunoassay. Positive influenza specimens detected by the Anyplex II RV16 Detection kit were tested by real-time PCR. Results. Compared to that of multiplex RT-PCR (influenza A, n=88; influenza B, n=82; influenza-negative, n=145), the sensitivities of Alere i, Sofia, and Veritor for influenza A were 97.7%, 72.7%, and 71.6%, respectively, whereas for influenza B, the sensitivities were 96.3%, 80.4%, and 75.6%, respectively. The specificity of Alere i, Sofia, and Veritor was 100.0%. Conclusions. The clinical performance of Alere i Influenza A&B is satisfactory, with the advantage of a significantly shorter test time than other molecular assays. It is suitable for point-of-care testing and rapid influenza diagnostic tests because of its high sensitivity and specificity.
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