Total Platelet Transcriptomics and Its Network Analysis by RNA-Seq and miRNA-Seq and PCA Application in Essential Thrombocythaemia
- Authors
- Moon, Kyung Chul; Gim, Jeong-An; Kim, Dae Sik; Choi, Chul Won; Yoon, Jung; Yoon, Soo-Young
- Issue Date
- 2021
- Publisher
- KARGER
- Keywords
- MicroRNA; Essential thrombocythaemia; Transcriptome
- Citation
- ACTA HAEMATOLOGICA, v.144, no.3, pp.337 - 344
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACTA HAEMATOLOGICA
- Volume
- 144
- Number
- 3
- Start Page
- 337
- End Page
- 344
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/130086
- DOI
- 10.1159/000510459
- ISSN
- 0001-5792
- Abstract
- Differentiating the aetiology of thrombocytosis is limited yet crucial in patients with essential thrombocythaemia (ET). MicroRNAs (miRNAs) regulate haematopoiesis and lineage commitment; aberrant expression of miRNAs plays an important role in myeloproliferative neoplasms. However, the miRNA profile has been poorly explored in ET patients compared to patients with reactive thrombocytosis (RT). A total of 9 samples, including 5 ET patient samples, 2 RT patient samples, and 2 healthy control samples, were analysed in this study. We produced 81.43 million reads from transcripts and 59.60 million reads from small RNAs. We generated a comprehensive miRNA-mRNA regulatory network and identified unique 14 miRNA expression patterns associated with ET. Among the 14 miRNAs, miR-1268a was downregulated in ET and showed an inverse correlation with its 8 putative target genes, including genes associated with thrombus formation and platelet activation (CDH6, EHD2, FUT1, KIF26A, LINC00346, PTPRN, SERF1A, and SLC6A9). Principal component analysis (PCA) showed ET and non-ET groups well clustered in space, suggesting each group had a distinctive expression pattern of mRNAs and miRNAs. These results suggest that the significant dysregulation of miR-1268a and its 8 target genes could be a unique expression of platelet mi-RNAs and miRNA/mRNA regulatory network in ET patients.
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