Generation of Induced Nephron Progenitor-like Cells from Human Urine-Derived Cells
- Authors
- Gao, Wei-Wei; Zheng, Jie; Yun, Wonjin; Kang, Phil-Jun; Park, Gyuman; Song, Gwonhwa; Kim, In-Yong; You, Seungkwon
- Issue Date
- 12월-2021
- Publisher
- MDPI
- Keywords
- direct reprogramming; kidney; nephron progenitor cells; transdifferentiation; urine cells
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.24
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 22
- Number
- 24
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/135654
- DOI
- 10.3390/ijms222413449
- ISSN
- 1661-6596
- Abstract
- Background: Regenerative medicine strategies employing nephron progenitor cells (NPCs) are a viable approach that is worthy of substantial consideration as a promising cell source for kidney diseases. However, the generation of induced nephron progenitor-like cells (iNPCs) from human somatic cells remains a major challenge. Here, we describe a novel method for generating NPCs from human urine-derived cells (UCs) that can undergo long-term expansion in a serum-free condition. Results: Here, we generated iNPCs from human urine-derived cells by forced expression of the transcription factors OCT4, SOX2, KLF4, c-MYC, and SLUG, followed by exposure to a cocktail of defined small molecules. These iNPCs resembled human embryonic stem cell-derived NPCs in terms of their morphology, biological characteristics, differentiation potential, and global gene expression and underwent a long-term expansion in serum-free conditions. Conclusion: This study demonstrates that human iNPCs can be readily generated and expanded, which will facilitate their broad applicability in a rapid, efficient, and patient-specific manner, particularly holding the potential as a transplantable cell source for patients with kidney disease.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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