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A rapid quantitative on-site coronavirus disease 19 serological test

Authors
Lee, Jeong HoonBae, Pan KeeKim, HyunhoSong, Yoon JiYi, So YeonKwon, JungsunSeo, Joon-SeokLee, Jeong-minJo, Han-SangPark, Seon MeePark, Hee SueShin, Kyeong SeobChung, SeokShin, Yong Beom
Issue Date
1-11월-2021
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Keywords
SARS-CoV-2; COVID-19; POCT; Microfluidics; Immunoassay
Citation
BIOSENSORS & BIOELECTRONICS, v.191
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS & BIOELECTRONICS
Volume
191
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/135796
DOI
10.1016/j.bios.2021.113406
ISSN
0956-5663
Abstract
On-site severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) serological assays allow for timely in-field decisions to be made regarding patient status, also enabling population-wide screening to assist in controlling the coronavirus disease 2019 (COVID-19) pandemic. Here we propose a rapid microfluidic serological assay with two unique functions of nanointerstice filling and digitized flow control, which enable the fast/robust filling of the sample fluid as well as precise regulation of duration and volume of immune reaction. Developed microfluidic assay showed enhanced limit of detection, and 91.67% sensitivity and 100% specificity (n = 152) for clinical samples of SARS CoV-2 patients. The assay enables daily monitoring of IgM/IgG titers and patterns, which could be crucial parameters for convalescence from COVID-19 and provide important insight into how the immune system responds to SARS CoV-2. The developed on-site microfluidic assay presented the mean time for IgM and IgG seroconversions, indicating that these titers plateaued days after seroconversion. The mean duration from day 0 to PCR negativity was 19.4 days (median 20 d, IQR 16-21 d), with higher IgM/IgG titres being observed when PCR positive turns into negative. Simple monitoring of these titres promotes rapid on-site detection and comprehensive understanding of the immune response of COVID-19 patients.
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