Immunostimulatory Potential of Extracellular Vesicles Isolated from an Edible Plant, Petasites japonicus, via the Induction of Murine Dendritic Cell Maturation
- Authors
- Han, Jeong Moo; Song, Ha-Yeon; Lim, Seung-Taik; Kim, Kwang-Il; Seo, Ho Seong; Byun, Eui-Baek
- Issue Date
- 10월-2021
- Publisher
- MDPI
- Keywords
- Petasites japonicus; T cells; dendritic cells; extracellular vesicles; immunostimulatory
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.19
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 22
- Number
- 19
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/136126
- DOI
- 10.3390/ijms221910634
- ISSN
- 1661-6596
- Abstract
- Extracellular vesicles (EVs) have recently been isolated from different plants. Plant-derived EVs have been proposed as potent therapeutics and drug-delivery nanoplatforms for delivering biomolecules, including proteins, RNAs, DNAs, and lipids. Herein, Petasites japonicus-derived EVs (PJ-EVs) were isolated through a series of centrifugation steps and characterized using dynamic light scattering and transmission electron microscopy. Immunomodulatory effects of PJ-EVs were assessed using dendritic cells (DCs). PJ-EVs exhibited a spherical morphology with an average size of 122.6 nm. They induced the maturation of DCs via an increase in the expression of surface molecules (CD80, CD86, MHC-I, and MHC-II), production of Th1-polarizing cytokines (TNF-a and IL-12p70), and antigen-presenting ability; however, they reduced the antigen-uptake ability. Furthermore, maturation of DCs induced by PJ-EVs was dependent on the activation and phosphorylation of MAPK and NF-kappa B signal pathways. Notably, PJ-EV-treated DCs strongly induced the proliferation and differentiation of naive T cells toward Th1-type T cells and cytotoxic CD8(+) T cells along with robust secretion of IFN-gamma and IL-2. In conclusion, our study indicates that PJ-EVs can be potent immunostimulatory candidates with an ability of strongly inducing the maturation of DCs.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.