Site-Directed Mutagenic Engineering of a Bifidobacterium Amylosucrase toward Greater Efficiency of Turanose Synthesis
DC Field | Value | Language |
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dc.contributor.author | Jun, Su-Jin | - |
dc.contributor.author | Lee, Jung-A | - |
dc.contributor.author | Kim, Young-Wan | - |
dc.contributor.author | Yoo, Sang-Ho | - |
dc.date.accessioned | 2022-04-12T12:41:54Z | - |
dc.date.available | 2022-04-12T12:41:54Z | - |
dc.date.created | 2022-04-12 | - |
dc.date.issued | 2022-02-09 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/140122 | - |
dc.description.abstract | The aim of this study was to establish one of the most efficient biocatalytic processes for turanose production by applying a robust Bifidobacterium thermophilum (BtAS) mutant developed through site-directed mutagenesis. A gene encoding the amylosucrase of B. thermophilum (BtAS) was cloned and used as a mutagenesis template. Among the BtAS variants generated by the site-directed point mutation, four different single-point mutants (P200R, V202I, Y265F, and Y414F) were selected to create double-point mutants, among which BtAS(Y414F/P200R) displayed the greatest turanose productivity without losing the thermostability of native BtAS. The turanose yield of BtAS(Y414F/P200R) reached 89.3% at 50 degrees C after 6 h with 1.0 M sucrose + 1.0 M fructose. BtAS(Y414F/P200R) produced significantly more turanose than BtAS-wild type (WT) by 2 times and completed the reaction faster by another 2 times. Thus, turanose productivity (82.0 g/(L h)) by BtAS(Y414F/P200R) was highly improved from 28.1 g/(L h) of BtAS-WT with 2.0 M sucrose + 0.75 M fructose. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | NEISSERIA-POLYSACCHAREA | - |
dc.subject | CHEMOENZYMATIC SYNTHESIS | - |
dc.subject | SPECIFICITY | - |
dc.subject | THERMOSTABILITY | - |
dc.subject | IDENTIFICATION | - |
dc.subject | QUANTITATION | - |
dc.subject | ELONGATION | - |
dc.subject | EXPRESSION | - |
dc.subject | DESIGN | - |
dc.subject | GLUCAN | - |
dc.title | Site-Directed Mutagenic Engineering of a Bifidobacterium Amylosucrase toward Greater Efficiency of Turanose Synthesis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Wan | - |
dc.identifier.doi | 10.1021/acs.jafc.1c06126 | - |
dc.identifier.scopusid | 2-s2.0-85123985093 | - |
dc.identifier.wosid | 000762960100018 | - |
dc.identifier.bibliographicCitation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.70, no.5, pp.1579 - 1588 | - |
dc.relation.isPartOf | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.title | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.volume | 70 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1579 | - |
dc.citation.endPage | 1588 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | NEISSERIA-POLYSACCHAREA | - |
dc.subject.keywordPlus | CHEMOENZYMATIC SYNTHESIS | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | THERMOSTABILITY | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | QUANTITATION | - |
dc.subject.keywordPlus | ELONGATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | GLUCAN | - |
dc.subject.keywordAuthor | amylosucrase | - |
dc.subject.keywordAuthor | turanose | - |
dc.subject.keywordAuthor | sucrose | - |
dc.subject.keywordAuthor | Bifidobacterium thermophilum | - |
dc.subject.keywordAuthor | site-directed mutagenesis | - |
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