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Surface display of enzyme complex on Corynebacterium glutamicum as a whole cell biocatalyst and its consolidated bioprocessing using fungal-pretreated lignocellulosic biomass

Authors
Lee, M.-E.Ko, Y.J.Hwang, D.-H.Cho, B.-H.Jeong, W.-Y.Bhardwaj, N.Han, S.O.
Issue Date
2022
Publisher
Elsevier Ltd
Keywords
Cellulase; Cellulosome; Corynebacterium glutamicum; Isopropanol; N-Acetylglucosaminidase
Citation
Bioresource Technology, v.362
Indexed
SCIE
SCOPUS
Journal Title
Bioresource Technology
Volume
362
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/146001
DOI
10.1016/j.biortech.2022.127758
ISSN
0960-8524
Abstract
A novel whole cell biocatalyst using fungal-pretreated lignocellulosic biomass was developed by displaying the enzyme complex consisting of N-acetylglucosaminidase (cNAG) and endoglucanse E (cCelE) on Corynebacterium glutamicum, hereafter called mNC. mNC showed a maximum 4.43-fold cNAG and 2.40-fold cCelE activity compared to single enzyme-secreting C. glutamicum. mNC also showed the highest efficiency of sugar production in various types of cellulose and fungal-pretreated biomass. The growth of mNC was 5.06-fold higher than that of the control. Then, the ability of mNC to produce a valuable chemical was confirmed. mNC overexpressing isopropanol biosynthesis genes showed a maximum titer of 218.9 ± 11.73 mg/L isopropanol and maintained high efficiency for isopropanol production in the recycling test, which was 90.07 ± 4.12 % during 4 cycles. This strategy can be applied to the direct saccharification of fungal-pretreated lignocellulosic biomass efficiently leading to the production of valuable products in various industrial fields. © 2022 Elsevier Ltd
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