Cancer-protective effect of a synbiotic combination betweenLactobacillus gasseri505 and aCudrania tricuspidataleaf extract on colitis-associated colorectal cancer
DC Field | Value | Language |
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dc.contributor.author | Oh, Nam Su | - |
dc.contributor.author | Lee, Ji Young | - |
dc.contributor.author | Kim, You-Tae | - |
dc.contributor.author | Kim, Sae Hun | - |
dc.contributor.author | Lee, Ju-Hoon | - |
dc.date.accessioned | 2021-08-30T09:25:44Z | - |
dc.date.available | 2021-08-30T09:25:44Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-11-09 | - |
dc.identifier.issn | 1949-0976 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/51821 | - |
dc.description.abstract | Previously, a synbiotic combination of probioticLactobacillus gasseri505 (LG) and a new prebiotic,Cudrania tricuspidataleaf extract (CT) in fermented milk, designated FCT, showed anin vitroimmunomodulatory effect and antioxidant activity. Although synbiotic combination might have cancer-protective effects, these activities have not been fully validatedin vivo. Ten-week treatment of LG, CT, or FCT to azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated colorectal cancer (CAC) mouse model reduced both the incidence of colonic tumors and damage to the colonic mucosa effectively, suggesting a cancer-protective effect. To understand these, biomarkers associated with inflammation, colon barrier, apoptosis, and cancer cell proliferation were monitored in AOM/DSS group versus LG/CT/FCT groups. A synbiotic combination (FCT) down-regulated pro-inflammatory cytokines (TNF-alpha, IFN-gamma, IL-1 beta, and IL-6) and inflammation-associated enzymes (iNOS and COX-2), and up-regulated anti-inflammatory cytokines (IL-4 and IL-10). In addition, colon barrier experiment revealed that biomarkers of mucus layer (MUC-2 and TFF3) and tight junction (occludin and ZO-1) were up-regulated. Subsequent apoptosis experiment showed that pro-apoptotic factors (p53, p21, and Bax) were up-regulated and anti-apoptotic factors (Bcl-2 and Bcl-xL) were down-regulated. Furthermore, comparative metagenome analysis of gut microbiota revealed thatStaphylococcusdecreased butLactobacillus, Bifidobacterium, andAkkermansiaincreased, supporting their protective effects, accompanied by increased short-chain fatty acids (SCFAs). Taken together, the FCT administration showed cancer-protective effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota. Consequently, the synbiotic combination (FCT) could be a novel potential health-protective natural agent against CAC. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.subject | INFLAMMATORY-BOWEL-DISEASE | - |
dc.subject | OXIDE SYNTHASE ACTIVITY | - |
dc.subject | CHAIN FATTY-ACIDS | - |
dc.subject | I-KAPPA-B | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | CUDRANIA-TRICUSPIDATA | - |
dc.subject | MEMBRANE-PROTEIN | - |
dc.subject | BETA-CATENIN | - |
dc.subject | ANTIINFLAMMATORY DRUGS | - |
dc.subject | BARRIER DYSFUNCTION | - |
dc.title | Cancer-protective effect of a synbiotic combination betweenLactobacillus gasseri505 and aCudrania tricuspidataleaf extract on colitis-associated colorectal cancer | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Sae Hun | - |
dc.identifier.doi | 10.1080/19490976.2020.1785803 | - |
dc.identifier.scopusid | 2-s2.0-85088029974 | - |
dc.identifier.wosid | 000552083000001 | - |
dc.identifier.bibliographicCitation | GUT MICROBES, v.12, no.1 | - |
dc.relation.isPartOf | GUT MICROBES | - |
dc.citation.title | GUT MICROBES | - |
dc.citation.volume | 12 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | INFLAMMATORY-BOWEL-DISEASE | - |
dc.subject.keywordPlus | OXIDE SYNTHASE ACTIVITY | - |
dc.subject.keywordPlus | CHAIN FATTY-ACIDS | - |
dc.subject.keywordPlus | I-KAPPA-B | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | CUDRANIA-TRICUSPIDATA | - |
dc.subject.keywordPlus | MEMBRANE-PROTEIN | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | ANTIINFLAMMATORY DRUGS | - |
dc.subject.keywordPlus | BARRIER DYSFUNCTION | - |
dc.subject.keywordAuthor | synbiotics | - |
dc.subject.keywordAuthor | immune modulation | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | tight junction | - |
dc.subject.keywordAuthor | colorectal cancer | - |
dc.subject.keywordAuthor | microbiome | - |
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