Detailed Information

Cited 9 time in webofscience Cited 10 time in scopus
Metadata Downloads

Cancer-protective effect of a synbiotic combination betweenLactobacillus gasseri505 and aCudrania tricuspidataleaf extract on colitis-associated colorectal cancer

Authors
Oh, Nam SuLee, Ji YoungKim, You-TaeKim, Sae HunLee, Ju-Hoon
Issue Date
9-11월-2020
Publisher
TAYLOR & FRANCIS INC
Keywords
synbiotics; immune modulation; apoptosis; tight junction; colorectal cancer; microbiome
Citation
GUT MICROBES, v.12, no.1
Indexed
SCIE
SCOPUS
Journal Title
GUT MICROBES
Volume
12
Number
1
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/51821
DOI
10.1080/19490976.2020.1785803
ISSN
1949-0976
Abstract
Previously, a synbiotic combination of probioticLactobacillus gasseri505 (LG) and a new prebiotic,Cudrania tricuspidataleaf extract (CT) in fermented milk, designated FCT, showed anin vitroimmunomodulatory effect and antioxidant activity. Although synbiotic combination might have cancer-protective effects, these activities have not been fully validatedin vivo. Ten-week treatment of LG, CT, or FCT to azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated colorectal cancer (CAC) mouse model reduced both the incidence of colonic tumors and damage to the colonic mucosa effectively, suggesting a cancer-protective effect. To understand these, biomarkers associated with inflammation, colon barrier, apoptosis, and cancer cell proliferation were monitored in AOM/DSS group versus LG/CT/FCT groups. A synbiotic combination (FCT) down-regulated pro-inflammatory cytokines (TNF-alpha, IFN-gamma, IL-1 beta, and IL-6) and inflammation-associated enzymes (iNOS and COX-2), and up-regulated anti-inflammatory cytokines (IL-4 and IL-10). In addition, colon barrier experiment revealed that biomarkers of mucus layer (MUC-2 and TFF3) and tight junction (occludin and ZO-1) were up-regulated. Subsequent apoptosis experiment showed that pro-apoptotic factors (p53, p21, and Bax) were up-regulated and anti-apoptotic factors (Bcl-2 and Bcl-xL) were down-regulated. Furthermore, comparative metagenome analysis of gut microbiota revealed thatStaphylococcusdecreased butLactobacillus, Bifidobacterium, andAkkermansiaincreased, supporting their protective effects, accompanied by increased short-chain fatty acids (SCFAs). Taken together, the FCT administration showed cancer-protective effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota. Consequently, the synbiotic combination (FCT) could be a novel potential health-protective natural agent against CAC.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Sae Hun photo

Kim, Sae Hun
융합생명공학과
Read more

Altmetrics

Total Views & Downloads

BROWSE