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Anti-Inflammatory Effect of a Peptide Derived from the Synbiotics, Fermented Cudrania tricuspidata with Lactobacillus gasseri, on Inflammatory Bowel Disease

Authors
Ha, JimyeongOh, HyeminOh, Nam SuSeo, YeongeunKang, JoohyunPark, Min HeeKim, Kyung SuKang, Shin HoYoon, Yohan
Issue Date
4-7월-2020
Publisher
HINDAWI LTD
Citation
MEDIATORS OF INFLAMMATION, v.2020
Indexed
SCIE
SCOPUS
Journal Title
MEDIATORS OF INFLAMMATION
Volume
2020
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/54431
DOI
10.1155/2020/3572809
ISSN
0962-9351
Abstract
The objective of this study was to evaluate the effects of peptides derived from synbiotics on improving inflammatory bowel disease (IBD). Five-week-old male C57BL/6 mice were administered with dextran sulfate sodium (DSS) via drinking water for seven days to induce IBD (IBD group). The mice in the IBD group were orally administered with PBS (IBD-PBS-positive control), Lactobacillus gasseri 505 (IBD-Pro), fermented powder of CT extract with L. gasseri 505 (IBD-Syn), beta-casein: LSQSKVLPVPQKAVPYPQRDMP (IBD-Pep 1), or alpha(s2)-casein: VYQHQKAMKPWIQPKTKVIPYVRYL (IBD-Pep 2) (both peptides are present in the synbiotics) for four more days while inducing IBD. To confirm IBD induction, the weights of the animals and the disease activity index (DAI) scores were evaluated once every two days. Following treatment of probiotics, synbiotics, or peptides for 11 days, the mice were sacrificed. The length of the small and large intestines was measured. The expression of the proinflammatory cytokines IL-1 beta, IL-6, TNF-alpha, and COX-2 in the large intestine was measured. Large intestine tissue was fixed in 10% formalin and stained with hematoxylin and eosin for histopathological analysis. The body weights decreased and DAI scores increased in the IBD group, but the DAI scores were lower in the IBD-Pep 2 group than those in the IBD group treated with PBS, Pro, Syn, or Pep 1. The lengths of the small and large intestines were shorter in the IBD group than in the group without IBD, and the expression levels of the proinflammatory cytokines were lower (p < 0:05) in the IBD-Pep 2 group than those in the IBD-PBS-positive control group. In addition, histopathological analysis showed that IBD was ameliorated in the Pep 2-treated group. These results indicate that Pep 2 derived from alpha(s2)-casein was effective in alleviating IBD-associated inflammation. Thus, we showed that these peptides can alleviate inflammation in IBD.
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