Novel Exopolysaccharide Produced from Fermented Bamboo Shoot-IsolatedLactobacillus Fermentum
- Authors
- Thi Bich Thuy Do; Thi Ai Luyen Tran; Thi Van Thi Tran; Trung Hieu Le; Jayasena, Vijay; Thi Hong Chuong Nguyen; Chinh Chien Nguyen; Kim, Soo Young; Quyet Van Le
- Issue Date
- 7월-2020
- Publisher
- MDPI
- Keywords
- lactic acid bacteria; phenylalanyl-tRNA synthase gene; exopolysaccharide; gas-liquid chromatography-mass spectrometry; Lactobacillus fermentum; nuclear magnetic resonance spectroscopy
- Citation
- POLYMERS, v.12, no.7
- Indexed
- SCIE
SCOPUS
- Journal Title
- POLYMERS
- Volume
- 12
- Number
- 7
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/54869
- DOI
- 10.3390/polym12071531
- ISSN
- 2073-4360
- Abstract
- This study aimed at providing a route towards the production of a novel exopolysaccharide (EPS) from fermented bamboo shoot-isolatedLactobacillus fermentum. A lactic acid bacteria strain, with high EPS production ability, was isolated from fermented bamboo shoots. This strain, R-49757, was identified in the BCCM/LMG Bacteria Collection, Ghent University, Belgium by the phenylalanyl-tRNA synthetase gene sequencing method, and it was namedLb. fermentumMC3. The molecular mass of the EPS measured via gel permeation chromatography was found to be 9.85 x 10(4)Da. Moreover, the monosaccharide composition in the EPS was analyzed by gas chromatography-mass spectrometry. Consequently, the EPS was discovered to be a heteropolysaccharide with the appearance of two main sugars-D-glucose and D-mannose-in the backbone. The results of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy analyses prove the repeating unit of this polysaccharide to be [-> 6)-beta-D-Glcp-(1 -> 3)-beta-D-Manp-(1 -> 6)-beta-D-Glcp-(1 ->](n), which appears to be a new EPS. The obtained results open up an avenue for the production of novel EPSs for biomedical applications.
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Collections - College of Engineering > Department of Materials Science and Engineering > 1. Journal Articles
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