Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
- Authors
- Bang, Jinho; Park, Heesun; Yoo, Jihye; Lee, Donghyun; Choi, Won Il; Lee, Jin Hyung; Lee, Young-Ran; Kim, Chungho; Koo, Heebeom; Kim, Sunghyun
- Issue Date
- 29-6월-2020
- Publisher
- NATURE RESEARCH
- Citation
- SCIENTIFIC REPORTS, v.10, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- SCIENTIFIC REPORTS
- Volume
- 10
- Number
- 1
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/54961
- DOI
- 10.1038/s41598-020-67522-4
- ISSN
- 2045-2322
- Abstract
- The global burden of bone-related diseases is increasing in the aging society; thus, improved bone targeted imaging for their early identification and treatment are needed. In this study, we screened novel peptide ligands for hydroxyapatite, a major inorganic component of teeth and bones, and identified a peptide enabling in vivo bone targeting and real-time fluorescence bone detection. To isolate peptides highly specific for hydroxyapatite, we used negative and positive selection from a randomized 8-mer peptide phage library and identified hydroxyapatite-specific peptides (HA-pep2, HA-pep3, and HA-pep7). Among these three peptides, HA-pep3 showed the highest binding capacity and superior dissociation constant towards hydroxyapatite surfaces over time (similar to 88.3% retained on hydroxyapatite after two weeks). Furthermore, HA-pep3 was highly specific for hydroxyapatite compared to other calcium salt-based materials. Using this superior specificity, HA-pep3 showed higher accumulation in skull, spine, and joints in comparison with scrambled control peptide during real-time whole-body imaging. Ex vivo analysis of the major organs and bone from mice demonstrated that the fluorescence intensity in bone was about 3.32 folds higher in the case of HA-pep3 than the one exhibited by the scrambled control peptide. Our study identified a novel approach for targeting ligands for bone specific imaging and can be useful for drug delivery applications.
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Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
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