Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects
- Authors
- Kim, Sung Eun; Choi, Somang; Hong, Jae-Young; Shim, Kyu-Sik; Kim, Tae-Hoon; Park, Kyeongsoon; Lee, Suk-Ha
- Issue Date
- 1월-2020
- Publisher
- MDPI
- Keywords
- nanodiamonds; lactoferrin; anti-oxidant; anti-inflammatory; osteogenic differentiation
- Citation
- NANOMATERIALS, v.10, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- NANOMATERIALS
- Volume
- 10
- Number
- 1
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/58498
- DOI
- 10.3390/nano10010050
- ISSN
- 2079-4991
- Abstract
- The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 mu g)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H2O2 treatment and increased proliferation of cells after H2O2 treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3-E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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