PEGylated Bilirubin-coated Iron Oxide Nanoparticles as a Biosensor for Magnetic Relaxation Switching-based ROS Detection in Whole Blood
- Authors
- Lee, Dong Yun; Kang, Sukmo; Lee, Yonghyun; Kim, Jin Yong; Yoo, Dohyun; Jung, Wonsik; Lee, Soyoung; Jeong, Yong Yeon; Lee, Kwangyeol; Jon, Sangyong
- Issue Date
- 2020
- Publisher
- IVYSPRING INT PUBL
- Keywords
- Bilirubin nanoparticles; Biosensors; Iron oxide nanoparticles; Magnetic relaxation switching; Reactive oxygen species
- Citation
- THERANOSTICS, v.10, no.5, pp.1997 - 2007
- Indexed
- SCIE
SCOPUS
- Journal Title
- THERANOSTICS
- Volume
- 10
- Number
- 5
- Start Page
- 1997
- End Page
- 2007
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/59069
- DOI
- 10.7150/thno.39662
- ISSN
- 1838-7640
- Abstract
- Rationale: Magnetic relaxation switching (MRSw) induced by target-triggered aggregation or dissociation of superparamagnetic iron oxide nanoparticles (SPIONs) have been utilized for detection of diverse biomarkers. However, an MRSw-based biosensor for reactive oxygen species (ROS) has never been documented. Methods: To this end, we constructed a biosensor for ROS detection based on PEGylated bilirubin (PEG-BR)-coated SPIONs (PEG-BR@SPIONs) that were prepared by simple sonication via ligand exchange. In addition, near infra-red (NIR) fluorescent dye was loaded onto PEG-BR@SPIONs as a secondary option for fluorescence-based ROS detection. Results: PEG-BR@SPIONs showed high colloidal stability under physiological conditions, but upon exposure to the model ROS, NaOCl, in vitro, they aggregated, causing a decrease in signal intensity in T2-weighted MR images. Furthermore, ROS-responsive PEG-BR@SPIONs were taken up by lipopolysaccharide (LPS)-activated macrophages to a much greater extent than ROS-unresponsive control nanoparticles (PEG-DSPE@SPIONs). In a sepsis-mimetic clinical setting, PEG-BR@SPIONs were able to directly detect the concentrations of ROS in whole blood samples through a clear change in T2 MR signals and a 'turn-on' signal of fluorescence. Conclusions: These findings suggest that PEG-BR@SPIONs have the potential as a new type of dual mode (MRSw-based and fluorescence-based) biosensors for ROS detection and could be used to diagnose many diseases associated with ROS overproduction.
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Collections - College of Science > Department of Chemistry > 1. Journal Articles
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