Mst1-Deficiency Induces Hyperactivation of Monocyte-Derived Dendritic Cells via Akt1/c-myc Pathway
- Authors
- Cho, Kyung-Min; Kim, Myun Soo; Jung, Hak-Jun; Choi, Eui-Ju; Kim, Tae Sung
- Issue Date
- 11-9월-2019
- Publisher
- FRONTIERS MEDIA SA
- Keywords
- Mst1; dendritic cells; hyperactivation; Akt1; c-myc; GM-CSF
- Citation
- FRONTIERS IN IMMUNOLOGY, v.10
- Indexed
- SCIE
SCOPUS
- Journal Title
- FRONTIERS IN IMMUNOLOGY
- Volume
- 10
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/62882
- DOI
- 10.3389/fimmu.2019.02142
- ISSN
- 1664-3224
- Abstract
- Mst1 is a multifunctional serine/threonine kinase that is highly expressed in several immune organs. The role of Mst1 in the activation of dendritic cells (DCs), a key player of adaptive immunity, is poorly understood. In this study, we investigated the role of Mst1 in GM-CSF-induced bone marrow-derived DCs and the underlying mechanisms. Mst1(-/-) DCs in response to GM-CSF expressed higher levels of activation/maturation-related cell surface molecules, such as B7 and MHC class II than Mst1(+/+) DCs. Furthermore, the expression of proinflammatory cytokines, such as IL-23, TNF-alpha, and IL-12p40, was increased in Ms1(-/-) DCs, indicating that Mst1-deficiency may induce the hyperactivation of DCs. Additionally, Mst1(-/-) DCs exhibited a stronger capacity to activate allogeneic T cells than Mst1(+/+) DCs. Silencing of Mst1 in DCs promoted their hyperactivation, similar to the phenotypes of Mst1(-/-) DCs. Mst1(-/-) DCs exhibited an increase in Akt1 phosphorylation and c-myc protein levels. In addition, treatment with an Akt1 inhibitor downregulated the protein level of c-myc increased in Mst1-deficient DCs, indicating that Akt1 acts as an upstream inducer of the de novo synthesis of c-myc. Finally, Akt1 and c-myc inhibitors downregulated the increased expression of IL-23p19 observed in Mst1-knockdown DCs. Taken together, these data demonstrate that Mst1 negatively regulates the hyperactivation of DCs through downregulation of the Akt1/c-myc axis in response to GM-CSF, and suggest that Mst1 is one of the endogenous factors that determine the activation status of GM-CSF-stimulated inflammatory DCs.
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