Preparation of Nanoemulsions of Vitamin A and C by Microfluidization: Efficacy on the Expression Pattern of Milk-Specific Proteins in MAC-T Cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae-Il | - |
dc.contributor.author | Kim, Tae-Gyun | - |
dc.contributor.author | Lim, Dong-Hyun | - |
dc.contributor.author | Kim, Sang-Bum | - |
dc.contributor.author | Park, Seong-Min | - |
dc.contributor.author | Hur, Tai-Young | - |
dc.contributor.author | Ki, Kwang-Seok | - |
dc.contributor.author | Kwon, Eung-Gi | - |
dc.contributor.author | Vijayakumar, Mayakrishnan | - |
dc.contributor.author | Kim, Young-Jun | - |
dc.date.accessioned | 2021-09-01T12:24:23Z | - |
dc.date.available | 2021-09-01T12:24:23Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2019-07-02 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/64164 | - |
dc.description.abstract | In this study, we prepared stabilized vitamin A and C nanoemulsions, and investigated their efficacy on milk-specific proteins in bovine mammary epithelial cells (MAC-T). Emulsions of vitamin A (vit-A) and C (vit-C) were prepared using Lipoid S 75 and microfluidization. The particle size and polydispersity index (PDI) of nanoemulsified vit-A and vit-C were studied. The cytotoxic effect of nanoemulsion-free and nanoemulsified vit-A and vit-C was determined by an MTT assay. In addition, the efficacy of nanoemulsified vit-A and vit-C on the in vitro expression pattern of milk-specific proteins in MAC-T cells was investigated by quantitative RT-PCR. The results showed that the efficacies of stabilized nanoemulsions of vit-A and vit-C were 100% and 92.7%, respectively. The particle sizes were around 475.7 and 225.4 nm, and the zeta potentials were around -33.5 and -21.3 mV, respectively. The expression changes of alpha(s2)-, beta- and kappa-casein were higher in the presence of a stabilized nanoemulsion of vit-A, compared with nanoemulsion-free vit-A. Furthermore, the expression changes of alpha(s2)- and beta-casein were lower and that of kappa-casein was higher in the presence of a stabilized nanoemulsion of vit-C, compared with nanoemulsion-free vit-C. Thus, our findings demonstrate the efficacy of nanoemulsified vit-A and vit-C in changing the expression of milk-specific proteins in MAC-T cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | DELIVERY-SYSTEMS | - |
dc.subject | ASCORBIC-ACID | - |
dc.subject | DAIRY-CATTLE | - |
dc.subject | FOLIC-ACID | - |
dc.subject | CASEIN | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | BIOAVAILABILITY | - |
dc.subject | NUTRACEUTICALS | - |
dc.subject | MICROEMULSIONS | - |
dc.subject | NANOPARTICLES | - |
dc.title | Preparation of Nanoemulsions of Vitamin A and C by Microfluidization: Efficacy on the Expression Pattern of Milk-Specific Proteins in MAC-T Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Jun | - |
dc.identifier.doi | 10.3390/molecules24142566 | - |
dc.identifier.scopusid | 2-s2.0-85068940388 | - |
dc.identifier.wosid | 000482303000049 | - |
dc.identifier.bibliographicCitation | MOLECULES, v.24, no.14 | - |
dc.relation.isPartOf | MOLECULES | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 24 | - |
dc.citation.number | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | DELIVERY-SYSTEMS | - |
dc.subject.keywordPlus | ASCORBIC-ACID | - |
dc.subject.keywordPlus | DAIRY-CATTLE | - |
dc.subject.keywordPlus | FOLIC-ACID | - |
dc.subject.keywordPlus | CASEIN | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | BIOAVAILABILITY | - |
dc.subject.keywordPlus | NUTRACEUTICALS | - |
dc.subject.keywordPlus | MICROEMULSIONS | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordAuthor | nanoemulsion | - |
dc.subject.keywordAuthor | microfluidization | - |
dc.subject.keywordAuthor | vitamins | - |
dc.subject.keywordAuthor | casein | - |
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