Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex
- Authors
- Park, Byoung Kwon; Maharjan, Sony; Lee, Su In; Kim, Jinsoo; Bae, Joon-Yong; Park, Man-Seong; Kwon, Hyung-Joo
- Issue Date
- 30-Jun-2019
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Keywords
- B cell epitope; Lipoplex (O); MERS-CoV; Monoclonal antibody; Spike protein
- Citation
- BMB REPORTS, v.52, no.6, pp.397 - 402
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BMB REPORTS
- Volume
- 52
- Number
- 6
- Start Page
- 397
- End Page
- 402
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/64717
- DOI
- 10.5483/BMBRep.2019.52.6.185
- ISSN
- 1976-6696
- Abstract
- Middle East respiratory syndrome coronavirus (MERS-CoV) uses the spike (S) glycoprotein to recognize and enter target cells. In this study, we selected two epitope peptide sequences within the receptor binding domain (RBD) of the MERS-CoV S protein. We used a complex consisting of the epitope peptide of the MERS-CoV S protein and CpG-DNA encapsulated in liposome complex to immunize mice, and produced the monoclonal antibodies 506-2G10G5 and 492-1G10E4E2. The western blotting data showed that both monoclonal antibodies detected the S protein and immunoprecipitated the native form of the S protein. Indirect immunofluorescence and confocal analysis suggested strong reactivity of the antibodies towards the S protein of MERS-CoV virus infected Vero cells. Furthermore, the 506-2G10G5 monoclonal antibody significantly reduced plaque formation in MERS-CoV infected Vero cells compared to normal mouse IgG and 492-1G10E4E2. Thus, we successfully produced a monoclonal antibody directed against the RBD domain of the S protein which could be used in the development of diagnostics and therapeutic applications in the future.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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