Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly
- Authors
- Kim, Taegon; Moon, Dongseok; Park, Jin Hyuk; Yang, Heehong; Cho, Seongyeon; Park, Tai Hyun; Ahn, Dong June
- Issue Date
- 28-4월-2019
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- NANOSCALE, v.11, no.16, pp.7582 - 7587
- Indexed
- SCIE
SCOPUS
- Journal Title
- NANOSCALE
- Volume
- 11
- Number
- 16
- Start Page
- 7582
- End Page
- 7587
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/65961
- DOI
- 10.1039/c9nr00249a
- ISSN
- 2040-3364
- Abstract
- A new polydiacetylene lipid/human olfactory receptor nano-assembly was fabricated for the visual detection of an odorant for the first time. The assembly consisted of phospholipid-mixed polydiacetylenes (PDAs) and human olfactory receptors (hORs) in detergent micelles. To overcome the limitations of bioelectronic noses, hOR-embedded chromatic complexes (PDA/hORs) were developed, introducing PDAs that showed color and fluorescence transitions against various stimuli. The chromatic nanocomplexes reacted with target molecules, showing a fluorescence intensity increase in a dose-dependent manner and target selectivity among various odorants. As a result, a color transition of the assembly from blue to purple occurred, allowing the visual detection of the odorant geraniol. Through circular dichroism (CD) spectroscopy and a tryptophan fluorescence quenching method, the structural and functional properties of the hORs embedded in the complexes were confirmed. Based on this first work, future array devices, integrating multiple nano-assemblies, can be substantiated and utilized in environmental assessment and analysis of food quality.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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